AMAAS

Network Project of Application of Microorganisms in Agriculture and Allied Sectors(AMAAS)

The scheme ‘Application of microorganisms in agriculture and allied sectors (AMAAS)’ was approved by the Planning Commission in the X plan in the 35th EFC meeting of DARE/ICAR held on 31st March, 2006 vide letter No, 3-17/2005/1A.III dated 27.6.06 for a period of one year in Xth Plan period and continued in XIth plan period. The scheme was continued in the XIIth plan and consecutive period from 2017-2020.

AMAAS Themes and projects under each theme( X & 11^th Five Year Plans)

Theme 1: Microbial Diversity and identification

• Isolation, identification and molecular assessment of microbial diversity from selected districts of Indogangetic Plain
• Mapping, assessment of the geographical distribution and in vitro conservation of agriculturally important microorganisms of the Western Ghats of India
• Diversity analysis of microbes in extreme conditions: Development of Diagnostic kit for the identification of Bacillus and fluorescent Pseudomonas
• Diversity of agriculturally important microorganisms in the Western Ghats of Kerala
• Agriculturally important microorganisms from soils of rice-based cropping system from agro-ecological zones of east coast of India
• Microbial diversity analysis from different Brakish water system of East Coast of India
• Isolation of microorganisms from fermented dairy foods and sequencing of 16S rDNA for strain identification
• Strengthening, authentication and exploitation of mushroom biodiversity at the National Mushroom Repository for Human Welfare
• Exploring the bacterial diversity in Kutch eco-region of Gujarat for agricultural and industrial applications
• Isolation and characterization of Flavobacterium species from fish and Aquatic Environment
• Diversity of diazotrophic bacteria in arid zone soil under extreme environments
• Exploration and screening of microbial diversity of Bihar and their potential application
• Exploration and screening of bacterial diversity in North-East India and its potential application in biocontrol
• Collection, identification and characterization of microbial diversity of North Bengal
• Biodiversity, characterization & conservation of cyanobacteria of Indo-Burma biodiversity hotspots (NE Zone of India) for harnessing of value added products
• Exploitation of Plant Growth Promoting Rhizobacteria for Sustainable Agriculture
• Exploration and screening of Fungal diversity in North-East India and their Applications in Agriculture
• Exploration of plant pathogenic and antagonistic microbial resources associated with vegetable and spice crops of Andaman and Nicobar Islands
• Collection, mapping, assessment of the geographical distribution and in vitro conservation of agriculturally important microorganisms of the Western Ghats
• Isolation and characterization of microorganism from freshwater ecosystem
• Diversity analysis of diazotrophic bacteria from wheat cropping system of   different agroclimatic zones of Punjab
• Microbial Diversity and Identification: Fish Microbes
• Development of a library of putative probionts from freshwater environment belonging to the group Lactic acid bacteria for application in freshwater aquaculture system
• Diversity of lactic acid bacteria in fermented food and dairy products from food and dairy units located in Southern Rajasthan

Theme2: Nutrient Management, PGPR, Antagonists, Biocontrol Agent and Disease management

• Improving yields and nutrient uptake of selected crops through microbial inoculants in Vertisols of Central India
• Exploration, collection and characterization of some agriculturally important biocontrol agents suitable for disease management
• Application of AIMS for nutrient management and plant growth promotion in rainfed agro ecosystem
• Development of a cold tolerant phosphate solubilizing bacterial (PSB) inoculant
• Isolation, identification, evaluation and exploitation of PGPR for Spices
• Development and application of PGPR formulations for growth improvement and disease suppression in coconut and cocoa
• Microbial control of insect pests
• Developing PGPR consortia for enhanced crop and soil productivity of rice-wheat cropping system
• Structural and functional dynamics of the microbial isolates in biogeochemical cycling of C, N, P and S in rice ecosystem
• Nutrient Dynamics and Carbon Sequestration in Plant Mycorrhizal systems
• Harnessing agricultural beneficial microorganisms for production and protection of Sorghum and Rice
• Isolation, identification, evaluation and exploitation of microorganisms for management of important plant pathogen and having PGPR potential for vegetable crops
• Application of PGPRs, biofertilizers and biocontrol agents for disease management and sustainable production of Tuber Crops
• Plant growth promoting rhizobacteria (PGPR) for chickpea and pigeon pea
• Development of suitable formulations of potential bioagents for management of important diseases and pests of Sunflower, Safflower and Castor
• Bioprospecting for viticulturally important microorganisms
• Development of a library of putative probionts from Marine environment belonging to the Genus Pseudomonas, Micrococcus and Bacillus for application in mariculture system
• Microbial Phosphorus transformation in inland open water
• Basic and applied investigations on endophytic microorganisms in horticultural crops
• Harnessing arbuscular mycorrhizae for biofertilization in horticultural crops

Theme 3: Microbial management of Agrowaste, Bioremediation and microbes in PHT

• Accessing structural and functional shifts in soil microbial communities of paper mill effluent contaminated soils and utilization of dominant micropflora for crop growth promotion in these soils
• Bioremediation of Polycyclic aromatic hydrocarbons (PAH) through microbial consortia
• Genotyping and isolation of Sphingomonads from HCN contaminated agricultural soils and their application in bioremediation
• Refinement in indoor compost technology for white button mushroom using thermophilic organisms
• Optimization of parameters for utilization of paddy straw, kinnow pulp and pea pods for production of cellulases, ethanol and feed supplements
• Bioremediation of commonly used pesticides in tropical rice ecosystem
• Development of bacterial consortia for bioprocessing agricultural wastes and bioremediation of aquaculture effluents
• Microbial bioremediation of waste water for heavy metals
• Bioremediation of effluents from shrimp farms
• Assessment of nisin production in selected strains of LAB and market acceptability
• Fermented products from fruits, vegetables and cereals
• Utilization of fruit processing waste for obtaining value added products through fermentation

Theme 4: Microbial Management of Abiotic Stress

• Development of microbial consortium for alleviation of salt stress for growth and yield of wheat
• Isolation, inventorization and field assessment of agriculturally important microorganisms in the stress ecosystems of Karnataka
• Development of microorganism consortium to alleviate abiotic stresses like drought, high temperature and salinity in millets

Theme 5: Microbial Genomics

• Structural genomics of Mesorhizobium ciceri (Ca181)
• Genomic studies of uncultivated N2-fixing communities from Uttarakhand
• Utilization of Actinomycetes to alleviate salt and drought stress for cereal crops
• Structural genomics of Mesorhizobium ciceri (ca 181)
• Functional genomic analysis of plant growth promoting rhizobacteria (PGPR) fluorescent Pseudomonads
• Mining for genes involved in production of fungicidal compounds in Anabaena strains
• Cloning and characterization of insecticidal crystal protein Gene (cry genes) from Local isolates of Bacilllus thuringiensis Berliner active against Coleopteran, Dipteran and Lepidopteran insect pests

Theme 6: Microbial genome resource repository (MGRR)

• Microbial genome resource repository

Theme 7: Human resource development (HRD)

• Human resource development

Theme 8: Nodal Center

AMAAS Themes and projects under each theme( 12^th Five Year Plan)

Theme 1a: Microbial Diversity with Special Focus on Extremophiles and Unique Niches

• Microbial diversity analysis of extreme ecological niches
• Exploration of the endophytic microbial diversity in horticultural crops through metagenomics and cultivation
• Mapping of deep-sea and hypersaline microbiota and their characterization using meta-omics approaches for bioprospecting of novel genes
• Analysis of bacterial community and their functional diversity from rice rhizosphere employing genomic and  metagenomics approaches
• Microbial diversity of bio-films in dairy niche
• Functional characterization of salt tolerant bacteria using multi-omic approaches: Towards the development of likelihood model of microbial amelioration of salt stress in wheat crop
• Culturable and unculturable microbial diversity of insects and their role in Insecticide resistance and other fitness attributes
• Molecular characterization and exploration of rumen microbial diversity in Black Bengal goats at north eastern state of Tripura
• Microbial Metagenomic Analysis of Hot Spring Situated Atop The Himalayan Ranges at Manikaran, Himachal Pradesh, India.
• Diversity of arbuscular mycorrhizal fungi and dark septate endophytes in woody species and shifting cultivation crop of Manipur, North East India

Theme 1b: Microbial Diversity with Special Focus on Biomolecules, Industrially Important Enzymes

• Exploring myxobacteria from the North Western Himalayas for novel enzymes and secondary metabolites
• Exploration and mass culture of fresh water cyanobacteria for development of  nutraceutical feed for  freshwater fish hatchlings

Theme 1c:  Microbial Diversity with Special Focus on Bio prospecting for novel genes and alleles

• Reference and de novo draft Genome Sequencing of  potential AIMS and their functional annotation
• Biomolecules and industrially important enzymes from extremophilic bacteria
• Draft Genome Sequencing of  P-solublizing Pseudomonas striata PS27 and functional validation of MSP Genes
• Genomics and Proteomics of  N2-fixing cyanobacterium with special reference to discovering abiotic stress tolerant novel genes and protein
• Exploiting the diversity of extreme halophiles by functional and comparative genomics for isolating novel genes and alleles for affording salinity tolerance to crop plants
• Isolation and selection of naturally occurring and genetically modified cyanobacterial strains for their potential use in biodegradation of agricultural pesticides (organophosphorous)

• Bio prospecting for Microbial Products that effects cold alleviation and growthPathogenomics-Genome sequencing of Pucciniastriiformis

Theme 1d: Microbial Diversity with Special Focus on Microbes from farm, fisheries and animals

• Microbial management in freshwater aquaculture
• Archaea and actinobacteria in vertisols of central India – assessment of diversity, biogeochemicalProcesses and bio inoculant potential
• Mining the Metagenome for Bacterial Diversity in Extreme Areas of North-East   India
• Microbial Diversity of Glacier Sample and peat profile of Himalayan Region

Theme 2: Taxonomy, Identification and Diagnostics of AIMs

• Development of microarray based Gene Chip for fungal plant pathogens under the background of DNA barcodes using multilocus gene phylogeny
• Exploration of plant growth promoting rhizobacteria, antagonistic and plant pathogenic microbial resources from high altitude agro-climate/ cropping system of Jammu and Kashmir state for sustainable agriculture.
• Development of diagnostic kit for detection of Kernal Bunt and loose smut of wheat
• Development of detection kit  for  detection of mycotoxigenic molds in ground nut

Theme 3 : Plant Microbe interactions, Nutrient management, disease control and Agro waste management

• Investigating the potential of root-colonizing microbial population for stress tolerance in chickpea through proteomics
• Identification of high trehalose-producing soybean rhizobia and their integration with AM fungi for enhanced drought tolerance in soybean
• Mapping and creation of gene bank of arbuscular mycorrhizal fungi and their exploration for enhancing productivity of underutilized crops
• Development of practicable technologies for field level exploitation of broad spectrum biocontrol agents for amelioration of biotic/abiotic stresses in crops
• Development of PGPR Inoculant Bioformulations for Rhizosphere Management in Enhancing Biomass of Fodder Crops
• Exploring endophytic and rhizospheric microorganisms for management of abiotic stress (drought) in rainfed maize and groundnut
• Mass production of Bacillus thuringiensis (Bt) and Beauveria bassiana, formulation as oil based suspension concentrates singly and in combination and field evaluation
• Development of formulations of Beauveria bassiana, Metarhizium anisopliae, Lecanicillium spp and Paecilomycesfumosoroseus for management of certain sucking pests in vegetable crops
• Role of potential micro-organisms in seed and crop health of rice, wheat and mustard
• Development of effective salt tolerant microbes to mitigate salt stress for higher crop production in salt affected soils
• Production and formulation technology refinement of bacterial bio-agents for soil borne plant disease management under coastal and plain ecosystems
• Prospecting the potential of cyanobacteria based formulations as plant growth promoting and biocontrol agents in cereal-legume cropping system and selected vegetables
• Degradation and effective utilization of agrowastes through technologies involving Mushrooms or macrofungi
• Metagenomics and cultural approaches for identification of novel microbial genes/alleles and microbes for bioconversion of lignocellulosic biomass at extreme physiological conditions under low temperature.
• Development of Bioformulations with PGPR, PGPF and AMF for induction of resistance in cereals and pulses against fungal pathogens and  improvement of their health status
• IdentificationofSaltTolerant Microbes and Development of Dynamic Substrate for Cultivation of Commercial Crops in Sodic Soils
• Development of microbial consortia as biocontrol for corm rot in Crocus sativus
• Exploration of microbial diversity in Seed Spices through Metagenomics approaches for crop improvement.
• Formulation of Coleoptera active cry toxins of Bacillus thuringiensis
• Influence of Arbuscular Mycorrhizal Fungi and Rhizobia in Conferring Drought Toleranceto Soybean
• Exploitation of PGPR and biocontrol agents for integrated management of biotic stresses of chilli in irrigated and dry land ecosystem

Theme 4 : Microbe Based Green energy

• Synthetic biology and metabolic engineering opportunities for enhanced production of biofuels through microbes.
• Thermophilic fungi from diverse ecosystems and their potential for producing industrially important enzymes
• Whole Genome and Transcriptome sequencing of selected cyanobacterial genomes for identification of the role their genes in metabolic pathways related to bio-energy production
• Developing enzyme catalyzed- microbe mediated processes for biofuels from mango waste (peel and kernel)

Theme 5: MGRR

Theme 6: Coordinating Unit and Human Resource Development

AMAAS Projects 2017-2020

Theme1 : Microbial Diversity

• Microbial Diversity Analysis of Extreme/Unique  Ecological Niches
• Genomics– Mediated Taxonomic and Functional Analysis of Endophytic Microbiome in Horticultural Crops and Plant–Microbe Interaction Studies
• Evaluation of Halotolerant Rhizobium and PGPB Based Biomolecules for Alleviation of Drought and Salt Stress
• Exploitation of Endosymbionts of Insect Pests for Pest Management
• To Study the Microbial Diversity Associated with Fish Gut by Using Culture-Dependent and Independent Approaches
• Development of Effective Formulation of Ecto-, Arbuscular Mycorrhizal and Dark Septate Endophytic Fungi for Growth Enhancement of Important Woody Trees and Crop Species in Manipur
• Transcriptome Profiling for Functional Annotation of ExiguobacteriumProfundum PHM11 Genome and Prospecting for Abiotic Stress Amelioration
• Developing Microbial Technology for Production of Industrially Important Biopolymer and Biomolecules
• Functional Characterization of Desiccation and /or Salinity Stress Induced Hypothetical Proteins from Diazotrophic Cyanobacterium Anabaena PCC7120
• Exploiting the Diversity of Extreme Halophiles by Functional and Comparative Genomics for Isolating Novel Genes and Alleles for Affording Salinity Tolerance to Crop Plants
• Characterization and Immobilization of Pesticide Induced Protein(S) of Cyanobacterium Fischerella sp. for Bioremediation of Pesticide Contaminated Water Systems
• Evaluation of Soybean-Rhizobia Interaction under Elevated CO2 and Temperature to Develop Climate Ready Microbial Inoculants for Central India

Theme2 : Taxonomy, Identification and Diagnostics of AIMs

• Development of Gene-chip for Detection of Major Fungal Plant Pathogens
• Development of Diagnostic Kit for Detection of Karnal Bunt and Loose Smut of Wheat
• Development of Bio-Intensive Disease Management Schedule for Production of Disease Free and Residue Compliant Grapes

Theme3 : Plant Microbe Interactions, Nutrient Management, Disease Control and Agrowaste Management

• Unravelling Transgenerational Impact of Competent Rhizosphere Microbes in Chickpea under Fusarium Oxysporum f. Sp. Ciceris Challenge
• Evaluation of Drought-Tolerant Rhizobia with and without AM Fungi for Enhanced Productivity of Soybean
• Mapping and Creation of Gene Bank of Arbuscular Mycorrhizae and their Exploration for Enhancing Productivity of Underutilized Crops
• PGPR Inoculant Bioformulations for Rhizosphere Management in Enhancing Biomass of Fodder Crops
• Mass Production of Bacillus Thuringiensis (Bt) and Beauveria Bassiana, Formulation as Oil Based Suspension Concentrates Singly and in Combination and Field Evaluation
• Development of Formulations of B. bassiana, M. anisopliae and Lecanicillium spp. for Management of Certain Sucking Pests in Vegetable Crops
• Production and Formulation Technology Refinement of Bacterial Bio-Agents for Soil Borne Plant Disease Management under Coastal Ecosystems
• Prospecting the Potential of Cyanobacteria Based Formulations as Plant Growth Promoting and Biocontrol Agents in Cereal-Legume Cropping System and Selected Vegetables
• Microbial Interventions for Production of Enzyme Supplement for Animal Feed from Fruit and Vegetable Mandi Waste
• Harnessing the Rhizosphere Diversity with Dynamic Substrate to Induce Tolerance to Abiotic and Biotic Stress for Commercial Cultivation of Agri-Horticultural Crops in Partially Reclaimed Sodic Soils
• BIOCLAY- the Novel LDH Nanocarrier System in Increasing the Persistence of Bt  Toxins
• Influence of Arbuscular Mycorrhizal Fungi and Rhizobia in Conferring Drought Tolerance to Soybean
• Developing PGPR Consortia for Enhanced Micronutrient (iron and zinc) Uptake and Yield of Finger Millet (Eleusinecoracana) in Hilly Areas
• Development of Research Modules and Pipelines for Commercialization and Regulatory Requirements Concerning Bio-Pesticides

Theme 4 : Microbe Based Green Energy

• Synthetic Biology and Metabolic Engineering Opportunities for Enhanced Production of Biofuels through Microbes
• Developing Thermophilic Fungal Strains as Source of Cellulase and Auxiliary Enzymes for 2-G Ethanol from Lignocellulosics
• Evaluation of Jute and Mesta Biomass for Bioethanol Production Potential

Theme 5: MGRR

Theme 6: Coordinating Unit and Human Resource Development