Dr. Hillol Chakdar

Senior Scientist (Agricultural Microbiology) Microbial Technology Unit II ICAR-NBAIM Professional experience: Joined Agricultural Research Service (ARS) on 02-07-2012 Working as Scientist at ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau since 10-10-2012 to till date Research interest: Microbial diagnostics Microbial diversity and taxonomy Application of microbes for nutrient and abiotic stress management Algology Lab webpage: https://sites.google.com/view/hillolchakdarlab/home Contact details: Tel: 91-8765107799 E-mail: hillol.chakdar@icar.gov.in

Significant recognition (Awards/Fellowships/Technologies transferred/Patents etc.):

1. Awards

1. 1. Indian National Science Academy (INSA) Medal for Young Scientists 2022
   2. Young Scientist Award by Uttar Pradesh Academy of Agricultural Sciences (UPAAS) in 2020
   3. International Travel Support (ITS) from SERB, DST in 2014
   4. IARI Merit Medal (Gold medal) in Ph.D. in 2013
   5. IARI Merit Medal (Gold medal) in M.Sc. in 2010

2. Fellowships:

1. 1. DST-INSPIRE Fellowship in 2010;
   2. CSIR-UGC Junior Research Fellowship in 2008
   3. ICAR-Junior Research Fellowship in 2007
   4. CICS Travel Fellowship 2016

3. Technologies:

1. 1. Liquid bioformulation known as BioPhos comprising P solubilising bacteria Kluyvera cryocrescens
   2. Diagnostic marker for early detection of Alternaria
   3. Diagnostic marker for early detection of Rhizoctonia solani causing sheath blight of rice
   4. Diagnostic marker for early detection of Sarocladium oryzae causing sheath rot of rice

4. Patents/Copyrights granted:

1. 1. PROCESS FOR  OBTAINING  HIGH  PURITY  PHYCOCYANIN  FROM CYANOBACTERIA  (Inventors:    Pabbi, H  Chakdar and  R  Kumar,  Applicant:  ICAR)  Patent No. 398346
   2. A METHOD OF DETECTION OF ALTERNARIA SPP. AND USES THEREOF (Inventors: PL Kashyap,  H  Chakdar,  AK  Srivastava  &  S  Rai,  Applicant:  ICAR) .  Patent No. 416987
   3. Copyright of database for MGR Portal. Authors: Renu, H Chakdar, UB Singh, A Singh. Registration number L-78707/2018

 

5. Patents filed

1. 1. 1. Application number: 1170/DEL/2015; Title: A method of high throughput fungal DNA template preparation and uses thereof ; Inventors: Hillol Chakdar, PL Kashyap, Alok Kumar Srivastava, Pragya Saxena
      2. Application number: 202111020221; Title: A portable device to perform isothermal amplification; Inventors: Hillol Chakdar, Shaloo Verma, Prassan Choudhary, Anil Kumar Saxena, Bansh Narayan Singh, Sudipta Das, Dikchha Singh
      3. Application number: 202111020898; Title: Method for the detection of rice sheath blight causing pathogen; Inventors: Hillol Chakdar, Prassan Choudhary, Sanjay K Goswami, Anil Kumar Saxena

 

6. Completed projects: Institute: 02; External: 04; Contract research projects: 02

Externally funded and contract research projects

Title of project	Funding Agency	Total Approved cost (lakhs INR)	Role in Project	Current status of the project
Inventorization of microbe based technologies developed in NARS for their effective translation from lab to land	Department of Scientific and Industrial Research (DSIR)	20.58	PI	Completed
Development of Genechip for major fungal pathogens	ICAR	62.00	PI	On going
Biofortification of Wheat and Maize with Zinc and Iron Using Endophytic Microorganisms	National Agricultural Science Fund (NASF)	52.57	CCPI	Completed
CRP Genomics	ICAR	138.08	Co-PI	Ongoing
Evaluation and utilization of stress alleviating microbes for selected cereal and oil seed crops (Co-PI)	Unite Phosphorus Limited, Mumbai	20.0	Co-PI	Completed
Evaluation of semifinished biofertilizer Nemix_C for plant growth promotion under contained environmental conditions	Chris Hansen (Pvt.) Ltd., Bengaluru	17.64	Co-PI	Completed

 

7. Publications (no.s):

S. No.	Type of publication	No.s
1	Full length papers	65
2	Book Chapters	14
3	Technical bulletin	10
4	Popular article	3

 

List of 10 important research articles

1. Choudhary, P., Waseem, M., Kumar, S., Subbarao, N., Srivastava, S., & Chakdar, H*. (2023). Y12F mutation in Pseudomonas plecoglossicida S7 lipase enhances its thermal and pH stability for industrial applications: a combination of in silico and in vitro study. World Journal of Microbiology and Biotechnology, 39(3), 1-14. [IF: 4.253]
2. Choudhary P, Goswami SK, Chakdar H*, Verma S, Thapa S, Srivastava AK and Saxena AK. (2022). Colorimetric loop-mediated isothermal amplification assay for detection and ecological monitoring of Sarocladium oryzae, an important seed-borne pathogen of rice. Front. Plant Sci. 13:936766. [IF: 6.60]
3. Choudhary, P., Bhowmik, A., Verma, S., Srivastava, S., Chakdar, H*., & Saxena, A. K. (2022). Multi-substrate sequential optimization, characterization and immobilization of lipase produced by Pseudomonas plecoglossicida S7. Environmental Science and Pollution Research, 1-15. [IF: 5.190]
4. Verma, S., Kumar, M., Kumar, A., Das, S., Chakdar, H.*, Varma, A., & Saxena, A. K. (2022). Diversity of bacterial endophytes of Maize (Zea mays) and their functional potential for micronutrient biofortification. Current Microbiology, 79(1), 1-14. [IF: 2.343]
5. Singh, D., Das, M.K., Srivastav, G.P., Verma, S., Choudhary, P., Das, S., Bhowmik, A., Singh, J.P., Thapa, S., Kumar, M. and Chakdar, H.* 2022. Genetic Diversity and Anti-Oxidative Potential of Streptomyces spp. Isolated from Unexplored Niches of Meghalaya, India. Current Microbiology, 79(12), 379. [IF: 2.343]
6. Choudhary, P., Bhowmik, A., Chakdar, H.*, Khan, M. A., Selvaraj, C., Singh, S. K., … & Saxena, A. K. (2022). Understanding the biological role of PqqB in Pseudomonas stutzeri using molecular dynamics simulation approach. Journal of Biomolecular Structure and Dynamics, 40(9), 4237-4249. [IF: 5.235]
7. Shahid, M., Singh, B. N., Verma, S., Choudhary, P., Das, S., Chakdar, H*., … & Saxena, A. K. (2021). Bioactive antifungal metabolites produced by Streptomyces amritsarensis V31 help to control diverse phytopathogenic fungi. Brazilian Journal of Microbiology, 52(4), 1687-1699. [IF: 2.214]
8. Choudhary, P., Rai, P., Yadav, J., Verma, S., Chakdar, H.*, Goswami, S. K., … & Saxena, A. K. (2020). A rapid colorimetric LAMP assay for detection of Rhizoctonia solani AG-1 IA causing sheath blight of rice. Scientific reports, 10(1), 1-19. [IF: 4.996]
9. Choudhary, P., Das, S., Chakdar, H.*, Singh, A., Goswami, S. K., & Saxena, A. K. (2019). Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants. BMC biotechnology, 19(1), 1-10. [IF: 3.327]
10. Choudhary, P., Chakdar, H.*, Singh, A., Kumar, S., Singh, S. K., Aarthy, M., … & Saxena, A. K. (2019). Computational identification and antifungal bioassay reveals phytosterols as potential inhibitor of Alternaria arborescens. Journal of Biomolecular Structure and Dynamics. [IF: 5.235]

List of 10 important review articles

1. Kumar, M., Chakdar, H*., Pandiyan, K., Thapa, S., Shahid, M., Singh, A., … & Saxena, A. K. (2022). Bacterial chitinases: genetics, engineering and applications. World Journal of Microbiology and Biotechnology, 38(12), 252. [IF: 4.253]
2. Verma, Shaloo; Thapa, Shobit; Siddiqui, Nahid; Chakdar, Hillol*. (2022). Cyanobacterial secondary metabolites towards improved commercial significance through multiomics approaches. World Journal of Microbiology and Biotechnology, 38(6): Jan-22 [IF: 4.253]
3. Chakdar, H., Thapa, S., Srivastava, A., & Shukla, P. 2021. Genomic and proteomic insights into the heavy metal bioremediation by cyanobacteria. Journal of Hazardous Materials, 127609. [IF: 14.224]
4. Kalwani, M*, H Chakdar*, Amit Srivastava, Sunil Pabbi, and Pratyoosh Shukla. 2021. Effects of nanofertilizers on soil and plant-associated microbial communities: Emerging trends and perspectives. Chemosphere, 132107. [IF: 8.943]
5. Verma, Shaloo; Chakdar, Hillol*; Kumar, Murugan; Varma, Ajit; Saxena, Anil Kumar. 2021. Microorganisms as a Sustainable Alternative to Traditional Biofortification of Iron and Zinc: Status and Prospect to Combat Hidden Hunger. Journal of Soil Science and Plant Nutrition 21, 1700–1717 [IF: 3.35]
6. Choudhary, Prassan; Singh, Bansh Narayan; Chakdar, Hillol*; Saxena, Anil Kumar. 2021. DNA barcoding of phytopathogens for disease diagnostics and bio-surveillance. World Journal of Microbiology and  Biotechnology 37, 54 [IF: 4.253]
7. Chakdar, Hillol; Hasan, Mafruha; Pabbi, Sunil; Nevalainen, Helena; Shukla, Pratyoosh. 2020. High-throughput proteomics and metabolomic studies guide re-engineering of metabolic pathways in eukaryotic microalgae: a review. Bioresource Technology, p.124495. [IF: 11.889]
8. Saxena, AK; Kumar, M; Chakdar, H; Anuroopa, N; Bagyaraj, DJ. 2020. Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology, 128(6): 1583-1594 [IF: 4.059]
9. Saini, Dinesh Kumar; Chakdar, H., Pabbi, Sunil; Shukla, Pratyoosh. 2020. Enhancing production of microalgal biopigments through metabolic and genetic engineering. Critical Reviews in Food Science and Nutrition, 60(3):391-405 [IF: 11.208]
10. Chakdar, Hillol*; Kumar, Murugan; Pandiyan, Kuppusamy; Singh, Arjun; Nanjappan, Karthikeyan; Kashyap, Prem Lal; Srivastava, Alok Kumar. 2016. Bacterial xylanases: biology to biotechnology. 3 Biotech, 6(2):150 [IF: 2.893]

*indicates corresponding author